It would be very interesting to assess hyaluronan levels in patients and animal models infected with COVID-19. Based on pre-existing studies, we expect that hyaluronan will be elevated in the lung tissues of patients with ARDS.
Rush UMC has established a Biorepository from their COVID19 patients. I will inquire with the PI (Alan Landay) as to availability of samples. Are there any labs currently equipped with appropriate Biosafety environments/equipment and methodologies (ELISA/FACE/HPLC) to perform such assays? Please post, so I can pass on that information.
Tissues are going to be in very high demand despite the number of cases and deaths in the US. Despite having access to patient blood and some sputum, we have not been able to access lung tissue. But, we are currently working on acquiring post-mortem autopsy specimens from COVID19 patients at our institution and expect to have access to these samples in the lab before the end of May. Our collaborators have had success working with labs in NY, but they are not permitted to send samples out - all work on post-mortem lung had to be performed in NY.
Currently COVID19 samples are not a select agent at our institution and we have been able to perform ELISA and other biochemical assays on plasma, as well as flow cytometry on fixed PBMCs, platelets, etc from patients. Essentially, if we can perform the assay within a biosafety cabinet, we are able to do it.